Ant “Formicidae”

Sample information

Photos by: Iryna O.
Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University

One ant arthropod was collected under the tree around 5 p.m. and about 85 F outside.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae


Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: Made sure to crush all samples really well and used the entire body of the arthropods.  

Used a 2% agarose gel

  • used 4 ul of 6X loading dye

Gel electrophoresis lanes: 

1 – 10ul of 1 Kb Plus DNA 

2- (no sample) 

3 – 20 ul of Unknown Arthropod #1 (Mosquito)

4 – 20 ul of Unknown Arthropod #2 (Ant)

5 – 20 ul of Positive Drosophila Control 

6 – 20 ul of Negative Drosophila Control 

7 – 20 ul of Positive DNA Control  

8 – 20 ul of Water  

Analysis: Extraction was done properly because there are bands percent for the two arthropods and three of the controls. Lane 8 has no bands because it is a water sample. 


Wolbachia presence No
Confidence level High
Explanation of confidence level

There were no problems during the laboratory procedure, and all steps were followed due to the protocol. All of the controls showed the expected results. The size of the Ant sample band on the gel is around 728 bp concluding that it amplified for the Arthropods CO1 gene. Was not able to detect the Wolbachia in the Ant sample.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be negative for Wolbachia.
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