Sample information

One ant arthropod was collected under the tree around 5 p.m. and about 85 F outside.

Methods

DNA extraction: Made sure to crush all samples really well and used the entire body of the arthropods.  

Used a 2% agarose gel

• used 4 ul of 6X loading dye

Gel electrophoresis lanes: 

1 – 10ul of 1 Kb Plus DNA 

2- (no sample) 

3 – 20 ul of Unknown Arthropod #1 (Mosquito)

4 – 20 ul of Unknown Arthropod #2 (Ant)

5 – 20 ul of Positive Drosophila Control 

6 – 20 ul of Negative Drosophila Control 

7 – 20 ul of Positive DNA Control  

8 – 20 ul of Water  

Analysis: Extraction was done properly because there are bands percent for the two arthropods and three of the controls. Lane 8 has no bands because it is a water sample. 

Results

There were no problems during the laboratory procedure, and all steps were followed due to the protocol. All of the controls showed the expected results. The size of the Ant sample band on the gel is around 728 bp concluding that it amplified for the Arthropods CO1 gene. Was not able to detect the Wolbachia in the Ant sample.