|Captive / Cultivated?||Wild-caught|
|Group||Palm Beach Atlantic University|
I caught this Flying ant on the side of a building in a shaded area. It was around 1pm and the temperature was 86 degrees F. This arthropod was alone in the shade on the wall.
|Putative identification||Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius niger|
|Extraction kit||DNeasy (Qiagen) blood and tissue kit|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
DNA Extraction: The insects abdomen was pestled really well making sure the solution was a homogenous mixture with very fine particles.
Gel Electrophoresis: Used 6X loading dye and gel was ran at 100V.
1- Kb Ladder
2- Unknown Arthropod 1 (Fly)
3- Unknown Arthropod 2 (Ant)
4- Positive arthropod control
5- Negative arthropod control
6- Positive DNA control
Analysis: The 1Kb ladder worked, the first unknown had one band. The second unknown arthropod (Lasius niger) did not have a band. The grinding may not have been sufficient enough to obtain a high yield of DNA extracted. In the 4th well, there was 2 bands present in the positive arthropod which was expected. In the 5th well, there was one band arthropod for the negative arthropod control. In the 6th well, there was 2 bands as expected for the positive DNA control. In the 7th well, no bands were present as expected.
|Explanation of confidence level||
All methods were thoroughly carried out as done outlined in the DNA extraction protocol.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Lasius niger was found to be negative for Wolbachia.|