Portland State University – Vespula

November 1, 2023

Sample information
Picture
Location
Collection date
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 11 is positive for COI, Lane 12 is a Wolbachia positive control, Lane 13 is blank for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
Report Inappropriate Post