Woodland Ground Beetle – Riya

November 17, 2023

Sample information
Picture
Photos by: Riya D.
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The beetle is small, black, and about 1/4 inch long. It was found in a slightly damp, tree covered dirt collection. It was very still until disturbed by outside forces and became very squirmy. The beetle has a noticeable pair of antennae on their head.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain
Gel images
Protocol notes
  • i had to dissect only the abdomen of the beetle to obtain the reproductive organs due to the hard shell cover of the arthropod
  • PCR and gel electrophoresis was somewhat successful. There are some visible bands, but the information is unclear. We were unable to send our samples in.

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level
  • The researched percentage of woodland ground beetles being infected with Wolbachia is very low, so I think that this organism does not have it. Although, it is still unknown.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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