2-SK

March 5, 2024

Sample information
Picture
Photos by: Sylvia K.
Location
Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

This arthropod has nineteen black spots on a pair of red-orange wings covering a hard, oblong body. It has a black head with white markings. It has six legs and is about a centimeter in length.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit Edwards Buffer
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

The results of all four of the controls were as expected: there was no contamination in the control with only water, the CO1 gene was visible in the other three controls, and the 16S rRNA gene was visible in the positive Wolbachia controls (refer to wells four to seven in image one and wells six to nine in image two). These results ensured there was no DNA contamination, and DNA extraction and PCR worked. While all of the controls worked, neither the CO1 gene nor the 16S rRNA gene were amplified in specimen 2-SK (refer to well three in image one and wells four and five in image two). Since the CO1 gene was not amplified, there was most likely an error in extracting the DNA. Nothing can be concluded about the possibility that 2-SK was infected with Wolbachia. 
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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