Austrosciara hyalipennis SM2

May 2, 2024

Sample information
Picture
Photos by: Saskia M.
Location
Collection date 03/07/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Fundort Trichocera hiemalis

auf dem Boden unter einem Blatt
Putative identification Arthropoda Insecta Diptera Trichoceridae Trichocera Trichocera hiemalis

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Confidence level high because the Cytochromoxidase bp is available and the bp for wolbachia is available.
Wolbachia 16S sequence Download FASTA    Download AB1
GACCAAATTTATAAKGTAATTGWAACTGCTCATGCTTTWATTATAATTTTTTTTATAGKAATACCTATWATAATTGGAGGATTTGGGAACTGATTAGTTCCCCTKATATTAAGAGCACCTGAKATAGCTTTCCCACGATTAAACAAWATAAGATTTTGACTTCTTCCTCCTTCATTAACATTATTATTAACTAGAAGATTAGTAGAAAGAGGAACAGGRACTGGRTGAACAGTKTACCCGCCTTTATCTWCTACTTTGYCTCATTCTGGCGCTTCAGWAGACCKTTCWATTTTCTCACTTCATTTAGCTGGWATCTCWACTATTTTAGGGGCAGTAAATTTTATTKCTACAATTATTAAYAYACSAMCCCCAGGAATATCTTTTGATAAAATA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ATCACTTAGAATATTAATCCGGGCAGAATTAGGTTGCCCTAATGCTTTAATTGGGGATGACCAAATTTATAATGTAATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGGGAACTGATTAGTTCCCCTTATATTAAAGAGCACCTGAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Trichocera hiemalis was found to be postive for Wolbachia.
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