Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence |
CTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Joro Spider
Imported Red Fire Ant
Black carpenter ant
Flocculent tailed millipede
Tawny crazy ant