GRP10 Coccinellidae (Ladybug Larvae)

May 25, 2024

Sample information
Picture
Location
Collection date 05/14/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations
  • Alone
  • In dry land
  • Shady areas filled with bushes
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit tweezers
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain UView
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Although we had sterile technique, multiple people in the lab were using the same materials like ladders, buffer, dyes, etc. which could’ve eventually led to some type of mishap in the lab. The controls did work so we know we can trust the results for the most part.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be postive for Wolbachia.
Report Inappropriate Post