Hoverfly

June 3, 2024

Sample information
Picture
Photos by: Senibua Sander
Location
Collection date 11/10/2023
Captive / Cultivated? Wild-caught
Group Kohler High School
Observations

It looked like a bee

Looked weak when caught, most likely due to cold weather

Found on doorbell
Putative identification Arthropoda Hexapoda Insecta Diptera Syrphidae

Methods
Extraction kit tweezers
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Took about three class periods (5 and a half hours)

Arthropod Image:

Well 1: Hoverfly

Well 2: Boxelder Bug

Well 3: Positive Arthropod control

Well 4: Negative Arthropod control

Well 5: Positive DNA control

Well 6: Water

Well: DNA Ladder

 

Wolbachia Image:

Well 1: DNA Ladder

Well 2: Hoverfly

Well 3: Boxelder bug

Well 4: Positive Arthropod Control

Well 5: Negative Arthropod Control

Well 6: Positive DNA Control

Well 7: Water

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We did this lab with the best care and followed every step to the T, so I believe our results are definitely true.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Syrphidae was found to be postive for Wolbachia.
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