Conura

June 4, 2024

Sample information
Picture
Photos by: Kaylie G.
Location
Collection date 05/28/2024
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

Has a stinger, seems to be a type of wasp, lives in a terrestrial habitat
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Chalcididae Conura

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

DNA extraction (Cell Lysis, Purification, Elution), PCR, Gel Electrophoresis, PCR Purification, Sequencing

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

During the gel electrophoresis, my arthropod DNA had issues with cross-contamination and was ruined – we did the gel but simply did not get a good sequencing reaction. In addition, the gel electrophoresis for my arthropod DNA had a very faint line while testing for Wolbachia, so I was scared that my arthropod didn’t have it and the band was a result of contamination from the other wells. However, I ended up getting a great result.
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTTACGNTNCGAATTCGTTTCNTTAGNGCTNATNNAGNNGGNTTTNCGNTTGACNNCTTTAAANCTNNNANGTNACGTTGCANNGNNTAGCGGTAGCCTGTTAACCAANNNGGTNTCTCNNCGCTGATCAAAAGNTNTTCCNATNNAATCNATCNGNNTAGNNATGACTNTGCGNNCGNGTNCNANAATACACTNTGAGNNATGAGAGCCTNAAGGAACANGATTTTTATCNATACTTGGTATCTCNCNACCN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Conura was found to be postive for Wolbachia.
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