Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | Forest path soil, near mixed forest foliage |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The gel image shows that no Wolbachia is present. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GGAATCTCTTCTATTTTAGGAGCTATCAATTTTATTTGTACTATCCTAAATATAATACCTCATAATATAAAAATTAATCAAATCCCCCTATTCCCATGATCAATCTTAATTACAGCAACTTTATTAATTTTATCATTACCAGYTTTAGCTGGTGCAATTACTATATTATTAACTGATCGAAACCTAAATACATCTTTCTTTGACCCATCAGGAGGAGGAGATCCAATTTTATATCAACATCTATTCTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Spotted cucumber beetle (Diabrotica undecimpunctata) C-5 SSP
DKH10
DKH 9
DKH8
DKH 7