DKH 9

November 21, 2024

Sample information
Picture
Location
Collection date 08/29/2024
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations

A very microscopic mite colored a reddish orange with long two front legs located on the front and back of it.

Was not able to get a picture due to the microscope preventing a photo from properly being taken.
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

While no Wolbachia DNA was found in the gel, the DNA quality after sequencing the specimen was very poor and could indicate some error.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TnnCnnCnnGnnnnnnnnnAnnnGGGnnnGCnnTnnnnnnnnnTnTTCnnnnnGnnnnnnnnnnACnnnATCTTnnnnCnnAnAnnAnnnnnTTTACnnnnnnnnnnTnACnGCnnnnnnnnTTATCAnnnnTTTCnTTATGGnnnnnnnnGCnnTnnnnnnnnnTnnnnnnnAnnnnnnTGnnnnnnnnAnnnnnnnnnnnnnnGATATnnnnnnCnnnCnnAnAAATAAnAnnnnnnTCnGATTACTACCCCCCTCTCnTAnACTTCTATTAnnCnnnnnnnTnnTCnnnnnnnnnnnnnGnnnnGGATGAAnTGnCnAnnnnnnnnnnnnnnGTnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnTTTnnnnnnnnnnnnnnnnnnGTAnnnnnnnnnnnnnnnnnnnnnnnTTTnnnnnnnnnnATnnnnnnTAnnnnAnnnnnnnnnnnnnnnnnnnnnnNnnnnCnnnnnnnnnnnnnnnnnNnnnnnnnnnnNnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnNNnnnnNNNNNnnG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arachnida was found to be negative for Wolbachia.
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