Triops

December 15, 2020

Sample information
Picture
Photos by: Elijah D.
Location
Collection date 11/19/2020
Captive / Cultivated? Captive / Cultivated
Group St. John High School USD 350
Observations

I purchased Triop eggs online and raised them. I was only able to get 3 to hatch out and those were the ones I extracted.
Putative identification Arthropoda Branchiopoda Notostraca

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The Triops were raised for about 2 weeks until they reached full size. They were then stored in 90% ethanol for a day until extraction.

DNA Extraction: The DNA was extracted from the abdomen.  All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.

PCR: Mini One Taq polymerase was used.

Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the triops only had one bright band that did not match up with my two banded positive controls. All of the protocols were also followed exactly so I am confident they are negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Notostraca was found to be negative for Wolbachia.
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