Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | The arthropod was feeding on a fellow student. It was late summer about 75 degrees Fahrenheit and 3:30 pm. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes albopictus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My gel and controls worked as expected and the results clearly showed that the insect was Wolbachia positive/ |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Aedes albopictus was found to be postive for Wolbachia. |
DEMAHF24-ACB Diptera
NIU21
NIU20
NIU19
NIU18