Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/21/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | Found in the forest |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | In both PCR processes strong results were observed. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | In both PCR processes there was a clear result. One was made by the students and the other one by our teacher. In both cases the lines are perfectly visible. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
Spotted cucumber beetle (Diabrotica undecimpunctata) C-5 SSP
DKH10
DKH 9
DKH8
DKH 7