Sample information

Caught in a pleasant park with many flowers ready for pollination. Caught during fall, with an outside temperature of 72 degrees Fahrenheit. Terrestrial habitat. Yellow and black, fuzzy, 6 legs, abdomen, 2 wings, disconnected head.

Methods

DNA Extraction: It took a while to crush up the abdomen. There was a lot of debris. Had to add more Buffer ATL because some of it spilled while grinding. Forgot to change pipette tips between samples.

PCR: Everything went according to the procedure. No errors made.

Gel Electrophoresis, Arthropod CO1: Lane 1 had the DNA ladder. Lane 2 had the DNA from our first arthropod, arthropod B-1. Lane 3 had DNA from arthropod B-2. Lane 4 had DNA from arthropod B-3. Lane 5 had our positive control. Lane 6 had our negative control. Lane 7 had H2O. The controls worked.

Gel Electrophoresis, Wolbachia: Lane 1 had the DNA ladder. Lane 2 had DNA from our first arthropod, artropod B-1. Lane 3 had DNA from arthropod B-2. Lane 4 had DNA from arthropod B-3. Lane 5 had our positive control. Lane 6 had our negative control. Lane 7 had H2O.

Results

Almost all labs went smoothly. However, when extracting DNA, pipette tips were not switched between samples, but there was no evidence of contamination. All controls worked during the gel electrophoresis, so any contamination was negligible.