Luna Moth-A1

November 19, 2024

Sample information
Picture
Photos by: Adrien P.
Location
Collection date 05/29/2024
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Location: Found in alley
Season: Spring
Temperature: 76°
Habitat: Urban

Description: Part of right wing is gone/has injured wing. Light green wings with two spots on each wing. Tan antennae, white torso, fuzzy texture.
Putative identification Arthropoda Insecta Lepidoptera Saturniidae Actias Actias luna

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

PCR: No mistakes made during PCR

Gel electrophoresis, Arthropod CO1:
Lane 1: Ladder
Lane 2: Arthropod A1 DNA
Lane 3: Arthropod A2 DNA
Lane 4: Arthropod A3 DNA
Lane 5: Positive Wolbachia Control
Lane 6: Negative Wolbachia Control
Lane 7: Water

No errors during the lab; our controls worked as intended

Gel Electrophoresis, Wolbachia:
Lane 1: Ladder
Lane 2: Arthropod A1 DNA
Lane 3: Arthropod A2 DNA
Lane 4: Arthropod A3 DNA
Lane 5: Positive Wolbachia Control
Lane 6: Negative Wolbachia Control
Lane 7: Water

No errors during the lab; our controls worked as intended

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

For both our arthropod and Wolbachia gel electrophoresis, our controls showed up as they should, which told us that our DNA was copied properly and there was no cross-contamination.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Actias luna was found to be negative for Wolbachia.
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