WRIAGF24-03 Orthoptera

November 6, 2024

Sample information
Picture
Photos by: Tamya Benniefield ; Contributed by David Almquist
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The nocturnal katydids use their green, leaf-like bodies to blend in during the day and frequently hide among thick foliage and trees. At sunset, they become more animated and talkative, particularly in hot, muggy conditions. Even though katydids can be difficult to spot, you can find them by listening for their distinctive, raspy sounds after nightfall. Try seeing them on branches with a flashlight or torch, and listen for movement when they call, as they are frequently found at heights of 6 to 15 feet. They will move more readily if you bring a tiny, foldable net or even just shake branches near their calls. This will make it easier to find and capture them.
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

“DNA extraction kit of in-house reagents was used.”

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I had strong confidence that my specimen would contain Wolbachia turns out it did not yikes. However , me and my lab partner successfully pushed through these long lab days and was able to get valid lab result which was still an amazing outcome.
Wolbachia 16S sequence
GTAGGCACATCATTAAGTATTTTAATTCGAACAGAACTAGGACAACCAGG CTATTTAATTGGAGATGATCAAACTTATAATGTTATCGTAACCGCACATG CATTTATCATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTT GGAAATTGATTAGTACCATTAATATTGGGGGCTCCAGATATAGCCTTCCC ACGAATAAACAATATAAGATTTTGACTTCTACCCCCATCATTAACCCTTT TATTAACCAGAAGAATAGTTGAAAATGGTGCAGGAACAGGATGAACAGTA TACCCACCTCTATCAACAGGAATCGCTCACGCGGGAGCATCCGTCGATTT AGCTATCTTCTCACTACATTTAGCAGGAATTTCCTCAATTCTAGGGGCTG TAAATTTTATCACTACCATAATTAATATACGAGCTCCAGGAATATCACTA GACCAAACACCATTATTTGTTTGAGCAGTCGGAATTACAGCTCTTCTATT ATTATTATCCTTACCAGTTTTAGCTGGTGCTATTACAATATTGCTTACAG ATCGAAATTTAAATACATCATTTTTTGATCCAGCGGGAGGAGGGGATCCA ATCTTATATCAACATCTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GTAGGCACATCATTAAGTATTTTAATTCGAACAGAACTAGGACAACCAGG CTATTTAATTGGAGATGATCAAACTTATAATGTTATCGTAACCGCACATG CATTTATCATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTT GGAAATTGATTAGTACCATTAATATTGGGGGCTCCAGATATAGCCTTCCC ACGAATAAACAATATAAGATTTTGACTTCTACCCCCATCATTAACCCTTT TATTAACCAGAAGAATAGTTGAAAATGGTGCAGGAACAGGATGAACAGTA TACCCACCTCTATCAACAGGAATCGCTCACGCGGGAGCATCCGTCGATTT AGCTATCTTCTCACTACATTTAGCAGGAATTTCCTCAATTCTAGGGGCTG TAAATTTTATCACTACCATAATTAATATACGAGCTCCAGGAATATCACTA GACCAAACACCATTATTTGTTTGAGCAGTCGGAATTACAGCTCTTCTATT ATTATTATCCTTACCAGTTTTAGCTGGTGCTATTACAATATTGCTTACAG ATCGAAATTTAAATACATCATTTTTTGATCCAGCGGGAGGAGGGGATCCA ATCTTATATCAACATCTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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