DEMAHF24-DSV (Hemiptera)

November 10, 2024

Sample information
Picture
Photos by: Daniel S.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Environment:
    – Grassy, open area.
    – Shrubs and trees.
    – Mildly hot weather.
    Collection Method:
    – Use a Sweep net .
    – Put them in a jar.
    – Ethanol Preservation
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My confidence level was medium because although I predicted that my insect might be infected with Wolbachia based on some initial signs or assumptions, I wasn’t fully certain. After analyzing data from different articles related to my Insect Order and even local data graphs, it became clear that Wolbachia was not going to be highly present in this insect species. This reflects the inherent uncertainty that can come with predicting the presence of an infection based on indirect or limited evidence.
Wolbachia 16S sequence
Arthropod COI sequence
AGCAGGTATAGTAGGATCCGCTATAAGACTAATTATCCGAATCGAACTAG GACAACCAGGAAGATTCATTGGAGATGATCAAATTTATAACGTAATCGTA ACAGCTCACGCCTTCGTAATAATTTTCTTCATAGTAATACCTATTATAAT TGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGCTCCCGACA TAGCATTCCCTCGTATAAATAACATAAGATTTTGATTATTACCTCCTTCA TTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCTGGAACCGG ATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCCCACAGAGGAGCAT CCGTAGACTTAGCAATTTTTTCACTCCACTTAGCCGGAGTATCTTCAATC TTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGACCCGCAGG AATAATCCCTGAACGAATTCCTTTATTTGTATGATCAGTAGGGATTACTG CCTTACTTCTACTACTATCATTACCTGTGCTAGCAGGTGCTATTACAATA TTATTAACTGATCGAAATTTTAACACATCATTTTTCGATCCCTCAGGTGG AGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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