Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Davies Career and Technical High School |
| Observations | The ant I collected was found in the woods in a decaying tree. It was similar to the Australian meat ant. I was unable to get a pictture of it. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level |
|
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
These are my results and I got all of the bands that were supposed to appear. I am confident that the ant I did has Wolbachia
MACALF24-GB Diptera
Lithobius forficatus A6
Wolbachia Project: Millipede
(no title)
Wolbachia Project #3 grasshopper