Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Found flying by a rotten log. Moved about for a while, but stayed around the log. Landed directly on my hand when I tried to catch it. It was in a group with other insects of the same species. Small, transparent wings, brown body, small brown legs, head is brown. |
| Putative identification | Arthropoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The band’s show that the controls and the experimental are flawed, indicating that there was an error in the conducting of our lab. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Because of the light and smudged bands (or the absence of bands all together), I am not confident in our results holding significance. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid