Carpenter Ant

September 11, 2023

Sample information
Picture
Photos by: Jennifer T.
Location
Collection date 10/02/2021
Captive / Cultivated? Wild-caught
Group William Fremd High School
Observations

This carpenter ant was found in the garage, and it was quick moving, yet easy to notice on the floor as it did not blend well with its surroundings when I located it.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

This is lane #146 in the given picture. There are multiple bands below the main band for the CO1 gene, so the gene may have been truncated, or there may have been nuclear genome repeats.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The controls worked.  A band was clearly visible in the proper region.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Hymenoptera was found to be postive for Wolbachia.
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