Diptera

March 28, 2021

Sample information
Picture
Location
Collection date 10/11/2020
Captive / Cultivated? Wild-caught
Group William Fremd High School
Observations

This insect was collected on a tall grass field.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

This was conducted in a lab.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There is a clean band that is very easy to identify. The two control bands worked well with no errors. The test were conducted by professionals.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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