Japanese Lady Bug

January 31, 2025

Sample information
Picture
Photos by: Meghan B.
Location
Collection date 11/07/2024
Captive / Cultivated? Wild-caught
Group John Bapst Memorial High School
Observations

The Japanese Lady Bug was small with a hard outer layer covering its abdomen. I extracted the abdomen of the lady bug for my PCR test.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes

During my lab procedure everything went as planned, there was no contamination and we got the expected results. The only problem that occurred was that we ran out of positive DNA for our 5th well so we didn’t get a positive DNA result.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I got the expected results of positive arthropod DNA and positive DNA as well as a negative negative control test. My confidence level is high because there was no contamination during the procedure so all of the results were high confidence.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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