Daddy Long Legs

January 31, 2025

Sample information
Picture
Photos by: Elmer Robichaud
Location
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group John Bapst Memorial High School
Observations

dead, very long legs, body and head seem to be as one.
Putative identification Arthropoda Chelicerata Arachnida Opiliones

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The sample showed a line under every part, meaning it was contaminated.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Opiliones was found to be negative for Wolbachia.
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