Ladybug LW

January 31, 2025

Sample information
Picture
Photos by: Laken W.
Location
Collection date 11/07/2024
Captive / Cultivated? Wild-caught
Group John Bapst Memorial High School
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes

My results were in the wells 2-7.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I got the anticipated response for my positive DNA and negative control, as well as positive results for my samples 1 and 2.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
Report Inappropriate Post