Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/26/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | The Grub was found under a Rock and was a really white and some what see through |
| Putative identification | Arthropoda Insecta Coleoptera Scarabaeidae Phyllophaga |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | wolbachia lab 1 then followed to lab 2 then to lab 3 then to lab 4. the only thing changed was the amout of agrose gel which was change to 40 ML |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | as the final results did not show complete accuracy so my confidence level was much lower and i feel like i may have not extrated enough DNA from the Grub |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Phyllophaga was found to be negative for Wolbachia. |
Bombus impatients (Eastern Bumblebee)
Agelenopis
My Data
Common Eastern Bumble Bee (Bombus impatiens)
American Bird