Sample information
Picture	Photos by: Aminah Mir, Chris Lebron ,Cecilia Tran
Location
Collection date	01/12/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	In kitchen
Putative identification	Arthropoda Insecta Diptera
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system Bio-Rad
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Diptera was found to be negative for Wolbachia.