Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 03/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | In leaf litter that was very moist and close to the soil. Temperature around 53 degrees fahrenheight. Description of organism is Pink-brownish centipede that is skinny. Has an orange hue towards the front end. Order Geophilomorpha. Scientific name identified as Strigamia bidens also known as the “Long Thin Centipede.” |
| Putative identification | Arthropoda Chilopoda Geophilomorpha Linotaeniidae Strigamia |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | No arthropod band was found and no Wolbachia band was found either. There is low confidence because there was no arthropod band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Strigamia was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid