Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/31/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | |
| Putative identification | |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Collected manually by hand in Aina loa, Hawai’i. Top Row C01(Wells 1-8) includes in order; DNA Ladder, Ant 1,Ant 2, Ant 3, Ant 4, Ant 5,Ant 6, Mosquito 1 (M1). Second row (Wells 9-18) includes in order; DNA Ladder, Ant 1,Ant 2, Ant 3, Ant 4, Ant 5,Ant 6, Mosquito 1 (M1). Third Row C01 Controls(Wells 16-21) includes in order; DNA ladder, DNA+, Diptera+, Diptera-, H20 Fourth Row 16S/Wolbachia Controls(Wells 25-29)includes in order; DNA ladder, DNA+, Diptera+, Diptera-, H20 |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All controls and samples were processed accordingly. Wolbachia was present for ant species but not in mosquito |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid