Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The reason I selected “Medium” for the confidence level is because my insect had a strong Wolbachia gel band, however, it had a BLAST match between 99.00% – 99.99% of 99.56%. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
TGGTTGTATCTCGGGGTTGACAGAACCGAAGAACGTATTCACCGTGGCGTGCTGACCACGATTCCTCGCGATTCAACTCCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGACCCTATCCCTTGACTGCGTATGCCAGAACCCGCTATCCCTGAGCCCCGCCTTATCGTAGGAAAATCATGTGCGCAAAAGGCCAGCAATAGGACAGGATACTATAAAAAGAATCGTTGTGGGAGTTGACCATTAGCTCCTCCCCCCTGACTAGGATCGATTACAATTAATCTTCCTCTATAAGTGGCGTATCCCTAAATGACTATAAAGATAGAAGGCGCTACCATCTGGACCCTCCCTCGT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
TATCGTTGGTTGATTCAGAATCGGGTCTCCCCTCCTGAAGGGATCAAAAAATGAAGTATTTAAGTTTCGATCAGTTAATAATATTCGTAATAGCTCCAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTCGCAGTGATTAAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTAAGGATTGTTGAAATAAAATTAATAGCTCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGCCCATTATGAAAAATATTTGAAGCTAAAGGAGGGTAGATAGTTCACCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTGATGGAAGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGCGATCCTAATATTAAAGGAATTAGAAAATTTCCAAATCCACCAATTATAAATGGTATTACTATAAAAAAGATTATAATAAAGGCATGATTAGTTACTAAAGAATTATAAATTTGATCATTATTAATTAATGAATTTGAAGAACCTAATTCTAATCGAATAATTATACTTATAGAAGATCCAATTATTCCAGCTCAGATAGCAAATAAAAAATAAAGAATTCCAATATCTTTATGTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Formicidae was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid