Sachem Skipper

April 28, 2025

Sample information
Picture
Photos by: Paige Springer
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

Wildflowers near Lake Mayes at Hampden-Sydney Campus

Season: Fall

Temperature: 78°F

Habitat: Terrestrial
Putative identification Arthropoda Insecta Lepidoptera Hesperiidae Atalopedes Atalopedes campestris

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

DNA extraction: The abdominal contents were removed and crushed thoroughly.

Gel electrophoresis lanes for CO1 arthropod and Wolbachia 16S rRNA PCR:

1: 1000bp ladder

2-7: other people’s DNA

8: arthropod primers

9: Wolbachia primers

10: control DNA

All of the controls worked.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band for Wolbachia , and there was a band for the arthropod lane. The controls also worked for everything else, making me confident that Wolbachia was absent.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Atalopedes campestris was found to be negative for Wolbachia.
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