Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/19/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | I found this the Pterostichus niger under a trunk in the forest near a path. I collected it in the afternoon, about 3pm. It was a sunny day and it was about 15 degrees outside. The area around the trunk was very dirty and wet. The Pterostichus niger was hiding in the dirt 5cm underneath the ground.
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| Putative identification | Arthropoda Insecta Coleoptera Carabidae Pterostichus Pterostichus niger |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | There is always the possibility to make mistake |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Pterostichus niger was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid