Armadillidium vulgare

May 23, 2025

Sample information
Picture
Photos by: Lauren W.
Location
Collection date
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

caught deceased.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
GTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTGGGGTTTGAGCAGGGGCTGTTGGGACTGCCCTTAGAATAATTATTCGCACTGAATTAGGACAGCCTGGAAGTTTAATTGGAGATGATCAGATTTACAATGTAATTGTAACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGTGGATTCGGTAACTGGTTAATTCCATTAATACTAGGAGCCCCAGATATAGCTTTTCCACGGATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACTTTATTACTTAGAAGGGGATTAGTTGAAAGAGGAGTAGGAACAGGGTGGACAGTATATCCTCCGCTGGCGTCGAGAATTGCTCACAGAGGTGCATCTGTAGATTTAGGTATTTTTTCTCTCCACCTTGCTGGAGCTTCTTCTATTTTAGGGGCTGTAAATTTTATTACTACTATAATTAATATACGAGCAGCTGGAATCAGAATAGACCGTGTTCCTTTATTTGTTTGATCAGTAATAGTAACGGCTGTGCTTTTGCTTTTATCATTACCTGTACTAGCAGGAGCTATTACTATATTATTAACTGATCGAAATTTAAATACCTCTTTTTTTGACCCTAGCGGAGGTGGGGATCCTATCCTTTATCAACATTTATTCTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium vulgare was found to be postive for Wolbachia.
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