northern flatid planthopper, Flatormenis proxima

September 11, 2025

Sample information
Picture
Photos by: Hong,Z
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found it on the leaves of the bushes. This insect prefers to fly and jump from one location to another.
Putative identification Arthropoda Insecta Hemiptera Flatidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Green
Gel images
Protocol notes

Lane 1 is the DNA ladder;

lane 2 is the target arthropod, siphanta acuta;

lane 3 is my colleague’s arthropod;

lane 4 is the positive wolbachia control;

lane 5 is the negative wolbachia control;

lane 6 is the wolbachia sampleDNA control (contain arthropod DNA);

lane 7 is the water control;

lane 8 is empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m confident in the results. Because the bonds (lane 2) are bright and easy to identify, the controlled group (lane 4-7) reconfirms the validation.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TGGATTTGATCNGGACTAGTAGGAATAATAATAAGAATAATTATTCGAATAGAACTATCTCAACCAGGATCA ATAATCAAAAATGACCAAATTTATAACACAATTGTAACTTCACATGCATTTGTAATAATCTTTTTTATAGTTATACCAAT TATAATTGGTGGTTTTGGAAACTGGCTTGTTCCTCTTATAATTGGAGCACCAGATATAGCTTTCCCACGAATAAACAATA TAAGTTTTTGACTTTTACCAATATCAATTACATTATTAATTGCAAGATCAACAGCAGCTTCAGGAACAGGAACAGGATGA ACTGTATACCCACCTCTTTCGGCTCAAGAAGCACATTCAGGACCATCAGTAGACTTAACAATTTTTTCATTACACATTGC AGGTATTAGATCAATTATAGGAGCAATCAATTTTATTTCAACCATTATAAATATACGAATCAAAGGAATAACAATAGAAA AAACCCCCTTATTTTGTTGATCAGTACTAATTACAGCAATCCTTTTACTAGTTTCTTTACCAGTACTAGCCGGAGCAATC ACAATATTAATTATAGACCGAAATTTAAATACTTCATTTTTTGATCCATCAGGAGGAGGTGATCCTATTCTGTATCAACA CTTATTCTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Flatidae was found to be negative for Wolbachia.
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