Lepidoptera (Moth)

September 11, 2025

Sample information
Picture
Photos by: Kiara Padilla
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Was seen together with a group. Seem attached to the flowers planted around the back of the campus.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain SYBR Green
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
NNNNNNNNNNNNNNNNNNNNNNNNNNTGANTGTCGTCANNTNANGNNGTGAGATGATTCGNTAGACCCACCTCGACCTCG ACCCTCATCCTTAAATGACATCATGTAATGCTGANCACTTCCTGCAAACTGCCANGGAATAATCTTANGANNANGGGGAN GATTATNNTNGGNGGGTNCGTTAAGAGATGTGTTCCACTTATGCTACAATGGCCTCTANNANGNNTCCCNNNNATAACAA NCATAANNTTTTCCCTAAANNCNNNTNGTTCGATTAGTACTCNNAATATCAACAGNNGANCTNGAANGGANAGGATGAAC NATNNACATGCCACTTNNNNNTTACGGGTCTTGGGACNTGNNGACTTGCCATGTTAATNGNNANACATTGCAGGTATTAG ATCAATTATAGGAGCAATTANTTTTATTTCTACCATTATAAATATGCNAATCAAGGGAATANCAATATAAAAAACCCCCT TATTTTGTTGATCAGTACTANTTACAGTAATCCTTTTACTATTTTCTTTATCAGTACTAGCCGTATCAATCTCAATATCA NTTATANACCTAAATCTAAATATNTTCATTTTTTGATCCATCANGANGACGCGANCCTATTCTGTATCANCNCTTATTNN GATTTTNNNNNNNNANNANNNTANAATN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TNNNNNNNNNNNNNNNTTTGATCNGGACAGTCGGANNNCANNAAGAATANTNATTCNANNAGAACTATCTCNTCCTGGAN CATTATTCGNAATGATCNATTTATAACTGTANTGTTCCTTCANNNGCATTTGGANTAATCNTTTTATNANTATNCNAATT ATAAGTGGTGGTTTNGGAAACTGACTTGTTCNNCTTATAATGGGAGCACCTGATATAGCTTTCCCACGAATAAACANTAT AAGNTTTTGACTTTNACCAATATTATTTACATTATTAATTGCAAGATCAACAGCATCTTCAGGAACAGGAACAGGATGAA CTGTATACCCTCCTCTTTCGGCTNNAGAACNTCTTTCAGGACCGTCAGTAGATTTAACAATTTTTTNNTTCCACATTGTA GGTATTATATCAATTATATGAGCAATTAATTTTATTTCGACCATTATNAATATGCGAATCAAGGGAATAACAATATAAAA AACCCCCTTATTTTGTTGATCAGTACTAATTACTGTAATCTTNTTNNATTTTCTTTACCNTTACTATCCGTAGCAANNNC TCTATTAGTTANATACCGAAATCTAAATANNTTNNTTTTTGATACATCANGAGGANGNGATCCTATACTGTATCANATTT TATTNTGATTTTTNNTNNNAAAAAAAAAAAANAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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