Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/08/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Galileo Academy of Science and Technology |
| Observations | |
| Putative identification | Arthropoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | BABEC |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Taq polymerase used Annealing temperature(s) for each PCR reaction – 55 degrees C |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Controls worked fine |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Bombus impatients (Eastern Bumblebee)
Agelenopis
My Data
Common Eastern Bumble Bee (Bombus impatiens)
American Bird