Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/29/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | When capturing My arthropod I noticed it was wrapped in Web. |
| Putative identification | Arthropoda Insecta Coleoptera Curculionidae |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We used a DNA extraction protocol based on the insects adaption of New England biolabs’ monarch spin gDNA extraction kit (product #T3010) The specimen was incubated for 30 minutes in a hot water bath at 56° C our first gel image was taken on October 21 and ran at 125 V for 20 minutes.we Used the DNA ladder : New England bio labs 1KB plus DNA ladder for safety stains product. #N0559S our second image was taken on November 4, 2025 and was running out 125 V for 25 minutes. We use this DNA ladder New England bio labs 1KB plus DNA ladder for safety stains product.# N0559S |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Confidence level high considering no band was present |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Curculionidae was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid