Roly poly

May 3, 2021

Sample information
Picture
Location
Collection date 05/31/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

It is brown and black. It has seven pairs of legs and two pairs of antennae. Its body is segmented.
Putative identification Arthropoda Crustacea Malacostraca

Methods
Extraction kit Edwards Buffer
DNA extraction location Other
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My pellet was visible and black and big.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Arthropod control  -A did not seem to work. -A and +A control in wolbachia gel did not seem to work.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Malacostraca was found to be postive for Wolbachia.
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