Roly poly

Sample information

Picture
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Ovular shape

Brown colored, lighter on the underside of the body

Scale-like divisions throughout the body

7 pairs of legs

1 set of antennae

No wings

Putative identification Crustacea Malacostraca Isopoda

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet had a small ovular shape. It’s color was light brown.

Results

Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

This is my first time extracting DNA, however I followed the protocols closely and everything went as planned.

Arthropod control -A didn’t seem to work

-A, +A in wolbachia gels didn’t seem to work

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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