Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/01/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Ovular shape Brown colored, lighter on the underside of the body Scale-like divisions throughout the body 7 pairs of legs 1 set of antennae No wings |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The pellet had a small ovular shape. It’s color was light brown. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | This is my first time extracting DNA, however I followed the protocols closely and everything went as planned. Arthropod control -A didn’t seem to work -A, +A in wolbachia gels didn’t seem to work |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be postive for Wolbachia. |
Water Boatman