May 3, 2021

Sample information
Picture
Photos by: Lauren A.
Location
Collection date 04/02/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

6 legs, segmented, wings, antenas, furry.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet was very large and dark.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My line was very faint and this is my first time extracting DNA.

Arthropod control -A didn’t seem to work. -A and +A control in Wolbachia gels didn’t seem to work.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be postive for Wolbachia.
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