Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/01/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | It was about a quarter of an inch long, had 6 legs, a small head, dark back, light belly, hard exoskeleton, and 2 leg like things at the bottom. I found it in a mound of dirt next to the wooden fence in my back yard. It was pretty wet and around 50 degrees that day. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The gels on the wolbachia test round were very shallow and crumbled a bit so we had to let them sit longer in the molds. My DNA pellet was small and visible, and dark brown. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Arthropod controls- not sure about the bands on walbachia dna, and negative arthropod. Walbachia controls- not sure about arthropod negative band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be postive for Wolbachia. |
Water Boatman