|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
It was about a quarter of an inch long, had 6 legs, a small head, dark back, light belly, hard exoskeleton, and 2 leg like things at the bottom. I found it in a mound of dirt next to the wooden fence in my back yard. It was pretty wet and around 50 degrees that day.
|Putative identification||Crustacea Malacostraca Isopoda|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
The gels on the wolbachia test round were very shallow and crumbled a bit so we had to let them sit longer in the molds. My DNA pellet was small and visible, and dark brown.
|Explanation of confidence level||
Arthropod controls- not sure about the bands on walbachia dna, and negative arthropod. Walbachia controls- not sure about arthropod negative band.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Isopoda was found to be postive for Wolbachia.|