Termite

May 4, 2021

Sample information
Picture
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

It was about a quarter of an inch long, had 6 legs, a small head, dark back, light belly, hard exoskeleton, and 2 leg like things at the bottom. I found it in a mound of dirt next to the wooden fence in my back yard. It was pretty wet and around 50 degrees that day.
Putative identification Arthropoda Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The gels on the wolbachia test round were very shallow and crumbled a bit so we had to let them sit longer in the molds. My DNA pellet was small and visible, and dark brown.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Arthropod controls- not sure about the bands on walbachia dna, and negative arthropod. Walbachia controls- not sure about arthropod negative band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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