Honey bee

May 4, 2021

Sample information
Picture
Location
Collection date 03/31/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Hybrid Russian honey bee, found outside hive, collected at about 5 pm, partly cloudy, 53 degrees
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Apidae Apis Apis mellifera

Methods
Extraction kit Edwards Buffer
DNA extraction location Partial abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Added more Edwards’s buffer because there was to little supernatant

Pellet: large, yellowish brown

gells were not properly prepared, the comb was inserted before gel was added, this led to inconsistently shaped wells.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Not sure if there was enough dna to process
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Apis mellifera was found to be negative for Wolbachia.
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