DEM-ATM Diptera

November 11, 2025

Sample information
Picture
Photos by: Amira M.
Location No map location data available.
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

In the grass with another one of its kind.

Bright orange thorax

 
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Instructor had a table of previous data of Georgia Southern students and their result of Wolbachia. My Order Diptera has an 18% chance of having Wolbachia which is pretty low. Also, the Gel electrophoresis showed no signs of my insect sample having Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
GTTGGAACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCATccctt ttttggtaTTGGTGATGATCAAATTTATAATGTAATTGTTAcatttcATG CTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTT GGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCC TCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTC TTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTA TATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTT AGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTG TTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTT GATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACT TCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAG ATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCT ATTTTATACCAACATTTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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