Ant

May 4, 2021

Sample information
Picture
Location
Collection date 03/29/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Medium sized black ant, two part body, black head and body, six light brown legs, found on the side of a large tree
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

There was a very small amount of visible pellet which was pale white/yellow, there could have been a miscalculation of the liquid in the cell lysis step the DNA could have been diluted

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The band is visible but faint.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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