Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/02/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | I noticed that the Ant was sort of clear looking under the microscope, had black eyes and looked like it had a flat bottom half. Another thing is that it had six legs, had a circle band in side of the stomach. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My pellet was brown, it was actually pretty big and noticeable, I disturbed it once so I had to restart that process but then I finally got it. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | It was low because my hand kept shaking while putting the liquid inside of the gel. I also Disturbed the pellet once. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Water Boatman