Roly Poly

May 4, 2021

Sample information
Picture
Photos by: Allison T.
Location
Collection date 10/02/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Small, black, flexible, found under rotting wood
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

I had accidentally taken out a piece of the arthropod when extracting the supernatant from the pellet. I had to extract all the DNA from the piece of the arthropod.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

 

First time doing the experiment
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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