Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/02/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Small, black, flexible, found under rotting wood |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | I had accidentally taken out a piece of the arthropod when extracting the supernatant from the pellet. I had to extract all the DNA from the piece of the arthropod. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level |
First time doing the experiment |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be postive for Wolbachia. |
Water Boatman