Roly Poly

Sample information

Photos by: Allison T.
Collection date 10/02/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

Small, black, flexible, found under rotting wood

Putative identification Arthropoda Crustacea Malacostraca Isopoda


Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

I had accidentally taken out a piece of the arthropod when extracting the supernatant from the pellet. I had to extract all the DNA from the piece of the arthropod.


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level


First time doing the experiment

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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