Roly Poly

Sample information

Picture
Photos by: Allison T.
Location
Collection date 10/02/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Small, black, flexible, found under rotting wood

Putative identification Crustacea Malacostraca Isopoda

Methods

Extraction kit Edwards Buffer
DNA extraction location Abdomen
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

I had accidentally taken out a piece of the arthropod when extracting the supernatant from the pellet. I had to extract all the DNA from the piece of the arthropod.

Results

Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

 


First time doing the experiment

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
Report Inappropriate Post