Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/20/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | -08/20/2025 :Wednesday –2:46 pm1 Location1 – Georgia Southern Armstrong Pond 1 Habitat 1 -Low tide pond1 Method of Collection: 1 -Dip Net 1 Method of Viewing :1 -Dissecting Magnification Glass1 – |
| Putative identification | Arthropoda Insecta |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in house raegents used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Based on the previous local data information the presence of a dipteran species was less than 20%. Then I extracted DNA , Sequenced the bases and compared the base sequences . I used Gel Electrophoresis, wherein I found that teh Wolbachia 16S Rrna gene was absent. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Melanoplus bivittatus – Xander Estacio
Bombus impatients (Eastern Bumblebee)
Agelenopis
My Data
Common Eastern Bumble Bee (Bombus impatiens)