Moth

May 5, 2021

Sample information
Picture
Location
Collection date 06/26/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The bug has a large abdominal area, a pair of antennae, 6 legs, and sturdy wings.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My pellet was barely visible after centrifuging the tubes for 5 minutes. In the moment I thought I had messed up somehow, but it turned out that the pellet was just a bright color, therefore making it difficult to see. I also feel as though I forgot to set the pipette to the right measurements when using it.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

DNA ladder in arthropod gel was not properly loaded. Positive and negative controls did not work. Wolbachia gel positive control did not work; there may have been problems with the DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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