Fruit Fly

Sample information

Photos by: Henry H
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
  • Has cool colors on the back
  • has wings
  • has 6 legs
  • has 3 parts to its body
  • has tiny hairs
Putative identification Arthropoda Hexapoda Insecta Diptera


Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  • Not sure if I got enough of the supernatant.
  • Not sure if I pipetted right into the gel
  • pellet was visible


Wolbachia presence No
Confidence level Low
Explanation of confidence level
  • DNA ladder in arthropod gel was not loaded properly
  • + and – controls didn’t work
  • wolbachia gel control didn’t work
  • there may be problems with the DNA extraction
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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