Fruit Fly

May 5, 2021

Sample information
Picture
Photos by: Henry H
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
  • Has cool colors on the back
  • has wings
  • has 6 legs
  • has 3 parts to its body
  • has tiny hairs
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  • Not sure if I got enough of the supernatant.
  • Not sure if I pipetted right into the gel
  • pellet was visible

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level
  • DNA ladder in arthropod gel was not loaded properly
  • + and – controls didn’t work
  • wolbachia gel control didn’t work
  • there may be problems with the DNA extraction
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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